FIG 7.

Contribution of T369I to NNRTI resistance. Site-directed mutants containing the T369I amino acid substitution, an amino acid substitution found in the connection domain of patient 12 (Fig. 6), in combination with Y181C were tested for susceptibility to ETR, RPV, EFV, and NVP. The lower cutoff values are indicated for each drug. Columns indicate mean values, while error bars indicate the standard errors of the means (SEMs). Each mutant was tested in duplicate in at least two independent experiments. ***, P < 0.001 (2-way analysis of variance).