FIG 7.

Identification of cytokines mediating the DMXAA-induced antiviral response. AML12HBV10 cells were cultured in the absence of tetracycline for 1 day either with or without preincubation with 10 μg/ml of a monoclonal antibody against type I IFN receptor IFNAR1 (Ab INFAR) at 37°C for 1 h, followed by treatment for 2 days with the concentrations of mouse IFN-α indicated (A) or 50% of the conditioned media harvested from RAW264.7 cells (treated with the indicated concentrations of DMXAA for 12 h) (B). Cytoplasmic HBV DNA were quantified by a real-time PCR assay, and data (mean values ± standard deviations; n = 4) are presented as percentages of the mock-treated control (NT) value. (C) AML12HBV10 cells cultured in the absence of tetracycline were treated for 4 days with the concentrations of IL-1, IL-6, or TNF-α indicated. Cytoplasmic HBV core DNA was analyzed by Southern blot hybridization. Lanes M contained molecular size markers.