FIG 2.

Direct binding between RSV604 and RSV N protein as measured by SPR analysis. (A, C, and E) Sensorgrams representing direct-binding kinetics for RSV604 against wild-type (wt) and mutant RSV N shown in response units (RU) as a function of time with increasing concentrations of RSV604. (B, D, and F) Plots of signals at equilibrium versus the concentrations of RSV604 to determine affinity. The data shown are from a representative SPR experiment using the indicated wt or mutant (L139I plus I129L or L139I) recombinant RSV N protein (amino acids 13 to 391).