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Effect of RSV604 on virus release and infectivity in HeLa cells. HeLa cells were infected with RSV A2 at an MOI of 0.1 in the presence of DMSO (control), RSV604 (1× EC90), or AZ-27 (1× EC90). (A) The culture supernatants containing the released virus were collected at the indicated times, and the amounts of released virus were measured by qRT-PCR for RSV RNA. (B) Released virus infectivity was determined by TCID50 assay. The data shown are means and SD (n = 2).
