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Frequency shifts of the QCM measurements of oritavancin binding to pure DOPC (A) or DOPC containing 0.1 mol% of lipid II derivative (B to E). The binding affinity KD results from the ratio of koff (afferent shift) and Kass (slope). (A) Intrinsic affinity of oritavancin to membranes; (B, C, and E) target binding by a much slower dissociation tendency, which is superimposed on membrane affinity.
