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. 1973 Jun;70(6):1757–1760. doi: 10.1073/pnas.70.6.1757

Replication of Bacteriophage ϕX-174 in a Mutant of Escherichia coli Defective in the dnaE gene

Lorance L Greenlee 1
PMCID: PMC433589  PMID: 4578442

Abstract

Strains of E. coli bearing a temperature-sensitive mutation at the dnaE locus were unable to support growth of bacteriophage ϕX-174 at 43° while permitting normal growth at 30°. The dnaE gene product was implicated in all stages of ϕX-174 DNA replication. Differences were observed between a double mutant (pol A-, dnaEts) and a single mutant (pol A+, dnaEts) in the ability to synthesize parental replicating form and its subsequent replication. The implications of these observations are discussed in terms of the relationship between DNA polymerases-I and -III.

Keywords: DNA polymerases, temperature-sensitive mutants, ϕX-174 DNA

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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