TABLE 3.

Effect of nitazoxanide-oseltamivir and nitazoxanide-oseltamivir carboxylate combination treatments in IAV-infected cells^a

Drug	Expt	MOI (PFU)	CI at indicated ED in:
			PR8-MDCK cells			WSN-A549 cells
			ED50	ED75	ED90	ED50	ED75	ED90
NTZ-OST	Single step	10	0.53	0.44	0.39	0.47	0.51	0.56
	Multistep	0.01	0.47	0.54	0.63	0.51	0.56	0.62
NTZ-OST-C	Single step	10	0.73	0.72	0.71	0.27	0.45	0.75
	Multistep	0.01	0.44	0.47	0.51	0.41	0.40	0.39

^aVirus yield was determined by HA titration at 24 h p.i. for single-step and 48 h p.i. for multistep experiments. Combination index (CI) values were generated by CalcuSyn software from duplicate samples from a representative experiment of three with similar results. NTZ-OST, nitazoxanide-oseltamivir phosphate; NTZ-OST-C, nitazoxanide-oseltamivir carboxylate.