Figure 5.
ScyA in excess affects the activity but not amount of the cbb3-HCO. (A) The activities of the cco promoter under indicated conditions determined by the integrative lacZ reporter. Error bars represent the SD of at least three independent experiments. (B) Production levels of the cbb3-HCO revealed by heme staining of CcoP. Proteins (10 μg per lane) extracted from indicated strains were separated on SDS-PAGE and analyzed by heme staining. The assays were repeated at least three times and similar results were obtained. HCO, heme–copper oxidases; SDS-PAGE, sodiumdodecyl sulphate polyacrylamide gel electrophoresis.