Table 1.
Strains and plasmids
| Strain or plasmid | Genotype or other relevant characteristics (name in figures) | Source or reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | Φ80dlacZΔm15, recA1, endA1, gyrA96, thi-1, hsdR17 (rk−, mk+), supE44, relA1, deoR, Δ(lacZYA-argF)U169 | Promega |
| Yarrowia lipolytica | ||
| W29 | MatA, wild-type | Barth and Gaillardin (1996) |
| Po1d | MatA ura3-302 leu2-270 xpr2-322 | Barth and Gaillardin (1996) |
| JMY330 | MatA ura3-302 leu2-270 xpr2-322, URA3ex; Ura+ Leu− | Beopoulos et al. (2008) |
| JMY2900 | MatA ura3-302 xpr2-322, URA3ex; Ura+ Leu+ | F. Brunel, (unpubl. data) |
| JMY2946 | Po1d ΔYALI0B19470::URA3ex Leu− | This study |
| JMY2949 | Po1d YALI0C21406::URA3ex YALI0C15488::LEU2ex (ΔYLjen1,2) | This study |
| JMY2956 | Po1d ΔYALI0C15488::URA3ex Leu− | This study |
| JMY3007 | Po1d ΔYALI0C21406::URA3ex Leu− | This study |
| JMY3012 | Po1d ΔYALI0D20108::URA3ex Leu− | This study |
| JMY3014 | Po1d YALI0C15488::URA3ex YALI0B19470::LEU2ex (ΔYLjen1,5) | This study |
| JMY3018 | Po1d YALI0C21406::URA3ex YALI0B19470::LEU2ex (ΔYLjen2,5) | This study |
| JMY3040 | Po1d YALI0E32901::URA3ex Leu− | This study |
| JMY3075 | Po1d YALI0D20108::URA3ex YALI0B19470::LEU2ex (ΔYLjen3,5) | This study |
| JMY3084 | Po1d ΔYALI0C21406 ΔYALI0C15488 Ura− Leu− | This study |
| JMY3086 | Po1d ΔYALI0C15488 ΔYALI0B19470 Ura− Leu− | This study |
| JMY3088 | Po1d ΔYALI0C21406 ΔYALI0B19470 Ura− Leu− | This study |
| JMY3090 | Po1d YALI0B19470::URA3ex YALI0E32901::LEU2ex (ΔYLjen5,6) | This study |
| JMY3092 | Po1d YALI0C15488::URA3ex YALI0E32901::LEU2ex (ΔYLjen1,6) | This study |
| JMY3093 | Po1d YALI0D20108::URA3ex YALI0E32901::LEU2ex (ΔYLjen3,6) | This study |
| JMY3094 | Po1d YALI0D20108::URA3ex YALI0C15488::LEU2ex (ΔYLjen1,3) | This study |
| JMY3101 | Po1d YALI0B19470::URA3ex + LEU2ex (ΔYLjen5) | This study |
| JMY3104 | Po1d YALI0C15488::URA3ex + LEU2ex (ΔYLjen1) | This study |
| JMY3106 | Po1d YALI0C21406::URA3ex + LEU2ex (ΔYLjen2) | This study |
| JMY3109 | Po1d YALI0D20108::URA3ex + LEU2ex (ΔYLjen3) | This study |
| JMY3112 | Po1d YALI0E32901::URA3ex + LEU2ex (ΔYLjen6) | This study |
| JMY3146 | JMY3084 + YALI0D20108::URA3ex Leu− | This study |
| JMY3147 | JMY3086 + YALI0D20108::URA3ex Leu− | This study |
| JMY3151 | JMY3084 + YALI0B19470::URA3ex Leu− | This study |
| JMY3152 | JMY3084 + YALI0B19470::URA3ex Leu− | This study |
| JMY3156 | JMY3088 + YALI0D20108::URA3ex Leu− | This study |
| JMY3171 | JMY3147 + YALI0E32901::LEU2ex (ΔYLjen1,3,5,6) | This study |
| JMY3209 | JMY3151 + LEU2ex (ΔYLjen1,2,5) | This study |
| JMY3210 | JMY3156 + LEU2ex (ΔYLjen2,3,5) | This study |
| JMY3213 | JMY3156 + YALI0E32901::LEU2ex (ΔYLjen2,3,5,6) | This study |
| JMY3215 | JMY3171 Ura− Leu− | This study |
| JMY3249 | JMY3156 + YALI0D24607::LEU2ex (ΔYLjen2,3,4,5) | This study |
| JMY3250 | JMY3151 + YALI0E32901::LEU2ex (ΔYLjen1,2,5,6) | This study |
| JMY3252 | Y3147 + YALI0D24607::LEU2ex (ΔYLjen1,3,4,5) | This study |
| JMY3257 | Po1d + YALI0D24607::LEU2ex Ura− | This study |
| JMY3278 | JMY3257 + URA3ex (ΔYLjen4) | This study |
| JMY3282 | JMY3215 + YALI0D24607::LEU2ex Ura− | This study |
| JMY3333 | JMY3282 + URA3ex (ΔYLjen1,3,4,5,6) | This study |
| JMY3598 | JMY3282 + YALI0C21406::URA3ex (ΔYLjen1-6) | This study |
| JMY3702 | JMY3598 Ura− Leu− | This study |
| JMY3722 | JMY3598 LEU2ex Ura− | This study |
| JMY3751 | JMY3722 + pTEF-YALI0B19470-URA3ex (YLJEN5++) | This study |
| JMY3754 | JMY3722 + pTEF-YALI0C15488-URA3ex (YLJEN1++) | This study |
| JMY3757 | JMY3722 + pTEF-YALI0D20108-URA3ex (YLJEN3++) | This study |
| JMY3760 | JMY3722 + pTEF-YALI0D24607-URA3ex (YLJEN4++) | This study |
| JMY3763 | JMY3722 + pTEF-YALI0E32901-URA3ex (YLJEN6++) | This study |
| JMY4125 | JMY3702 + pTEF-C21406-LEU2ex Ura− | This study |
| JMY4157 | JMY4125 + URA3ex (YLJEN2++) | This study |
| Plasmids | ||
| pCR4Blunt-TOPO | Cloning vector | Invitrogen |
| JMP802 | JMP62-pTEF-LEU2ex | |
| JMP803 | JMP62-pTEF-URA3ex | (Müller et al. 1998; Nicaud et al. 2002) |
| JMP547 | pUB4-CRE | Fickers et al. (2003) |
| JMP1392 | JMP62-pTEF-RedStar2SKL-LEU2ex | Kabran et al. (2012) |
| JMP1647 | pCR4Blunt-TOPO YALI0B19470UpDn | This study |
| JMP1649 | pCR4Blunt-TOPO YALI0C15488UpDn | This study |
| JMP1683 | pCR4Blunt-TOPO YALI0C21406UpDn | This study |
| JMP1684 | pCR4Blunt-TOPO YALI0D20108UpDn | This study |
| JMP1689 | pCR4Blunt-TOPO YALI0E32901UpDn | This study |
| JMP1667 | pCR4Blunt-TOPO YALI0B19470UpDn-URA3ex | This study |
| JMP1671 | pCR4Blunt-TOPO YALI0C15488UpDn-URA3ex | This study |
| JMP1679 | pCR4Blunt-TOPO YALI0B19470UpDn-LEU2ex | This study |
| JMP1681 | pCR4Blunt-TOPO YALI0C15488UpDn-LEU2ex | This study |
| JMP1698 | pCR4Blunt-TOPO YALI0C21406UpDn-URA3ex | This study |
| JMP1702 | pCR4Blunt-TOPO YALI0D20108UpDn-URA3ex | This study |
| JMP1713 | pCR4Blunt-TOPO YALI0E32901UpDn-URA3ex | This study |
| JMP1715 | pCR4Blunt-TOPO YALI0E32901UpDn-LEU2ex | This study |
| JMP 1792 | JMP62 pTEF-YALI0B19470-URA3ex | This study |
| JMP1794 | JMP62 pTEF-YALI0C15488-URA3ex | This study |
| JMP1796 | JMP62 pTEF-YALI0D24607-URA3ex | This study |
| JMP1798 | JMP62 pTEF-YALI0E32901-URA3ex | This study |
| JMP1809 | JMP62 pTEF-YALI0D20108-URA3ex | This study |
| JMP1813 | pCR4Blunt-TOPO YALI0D24607UpDn-LEU2ex | This study |
| JMP2366 | JMP62 pTEF-YALI0C21406-LEU2ex | This study |