Table 2.
Primer list
| Genes | Primers | Sequences | Utilization |
|---|---|---|---|
| YALI0B19470g (YLJEN5) | B19470UpNotI | GAATGCGGCCGCCAGTTGGTTCAACTGGGTCC | Upstream fragment of YALI0B19470 |
| B19470UpIsceI | CGATTACCCTGTTATCCCTACCGGCATGAGAGTGGTGTTTGG | ||
| B19470DnNotI | GAATGCGGCCGCCAGCCAGGTCAGCTACTATT | Downstream fragment of YALI0B19470 | |
| B19470DnIsceIIceuI | GGTAGGGATAACAGGGTAATCGTAACTATAACGGTCCTAAGGTAGCGACCAATCTCACTCGTTAATTAG | ||
| Ver1B19470 | CAAGTATCATGGTTTGTGGG | Verification of disruption | |
| Ver2B19470 | GTGTCACAACAGTGTCACCC | ||
| StartB19470 | ATCGGATCCCACAATGCCCATCACAGTTTCACAAGAAGTG | Overexpression of YALI0B19470 | |
| EndB19470 | CATCCTAGGTTAACGAGTGAGATTGGTGTCGATTCG | ||
| B19470F | TCTTCTTCTTGCAGTTCTTTG | Expression of YALI0B19470 by RT-PCR | |
| B19470R | GTGCTCATCGAGAATAGGG | ||
| YALI0C15488g (YLJEN1) | C15488UpNotI | GAATGCGGCCGCTTTGTCTGTCTTCCCGTTGC | Upstream fragment of YALI0C15488 |
| C15488UpIsceI | CGATTACCCTGTTATCCCTACCGGTGAATGAACGAACGAAGAAGG | ||
| C15488DnNotI | GAATGCGGCCGCTGAGAGTCCTTCTGTCTACC | Downstream fragment of YALI0C15488 | |
| C15488DnIsceIIceuI | GGTAGGGATAACAGGGTAATCGTAACTATAACGGTCCTAAGGTAGCGAGTGTAGCAACTAACGTATTG | ||
| Ver1-1C15488 | CCACTCCTCCTCTCCTTAGACCG | Verification of disruption | |
| Ver2-2C15488 | CCTCTCCATCGATTTCGAGGTGC | ||
| StartC15488 | ATCGGATCCCACAATGGATTTGGACAACCTCCCTGCCCC | Overexpression of YALI0C15488 | |
| EndC15488 | CATCCTAGGCTACTTAGTAGCATTGGTGTCAACTC | ||
| C15488F | TTCTTCTTGCAGTTCTTCGT | Expression of YALI0C15488 by RT-PCR | |
| C15488R | CGACCCCATTATCATCTTT | ||
| YALI0C21406g (YLJEN2) | C21406UpNotI | GAATGCGGCCGCGGAGAAAATGGACGTGTGAGACGC | Upstream fragment of YALI0C21406 |
| C21406UpIsceI | CGATTACCCTGTTATCCCTACCGAGATAGAGCAAGTAGAAGCAGCG | ||
| C21406DnNotI | GAATGCGGCCGCGGACTTGTAACAGCACACGTTCGC | Downstream fragment of YALI0C21406 | |
| C21406Dn2IsceIIceuI | GGTAGGGATAACAGGGTAATCGTAACTATAACGGTCCTAAGGTAGCGACTCTGAGACCCTCCCCGGATCCC | ||
| Ver1C21406 | CATTAGCGTGGTTTCCATGCG | Verification of disruption | |
| Ver2C21406 | AAGAGAGTTACTTTTGCGGGAGG | ||
| StartC21406 | ATCGGATCCCACAATGGATCTCGACAACTACCCTCCTC | Overexpression of YALI0C21406 | |
| EndC21406 | CATCCTAGGTCACTTTTGGGATCCGGGGAGGGTCTC | ||
| C21406F | GATCTTCCATGATTGGTATTG | Expression of YALI0C21406 by RT-PCR | |
| C21406R | CAGGGTGACGACAAAGAG | ||
| YALI0D20108g (YLJEN3) | D20108UpNotI | GAATGCGGCCGCGCACAAGTAGGTGGGTCCTTCCG | Upstream fragment of YALI0D20108 |
| D20108UpIsceI | CGATTACCCTGTTATCCCTACCGACAGATTGAGCGAGTAGACAGG | ||
| D20108DnNotI | GAATGCGGCCGCGAGAATCTGGAAGCATCCTGCTGCC | Downstream fragment of YALI0D20108 | |
| D20108Dn2IsceIIceuI | GGTAGGGATAACAGGGTAATCGTAACTATAACGGTCCTAAGGTAGCGATAATGTAGGGTGTGTGTGTGACG | ||
| Ver1-2D20108 | CCAAGGGTGGCCATTAGGAGTCG | Verification of disruption | |
| Ver2-2D20108 | GGTGTCATATTCGAGTCCTCGC | ||
| StartD20108-2 | ATCGGATCCCACAATGAATTTTGACAACTTCCCAGCACCAGATCTG | Overexpression of YALI0D20108 | |
| EndD20108-2 | CATCCTAGGTTATCGAGTATCGCTCGAAGAACGTCTTTCAATGTTC | ||
| D20108F | CTTCTTCTGCTTCTTCAACC | Expression of YALI0D20108 by RT-PCR | |
| D20108R | CATCTCTGCTTGTCTGTTTTC | ||
| YALI0D24607g (YLJEN4) | D24607UpNotI | GAATGCGGCCGCGATGCAGATCTTTCCGAGCGCTGG | Upstream fragment of YALI0D24607 |
| D24607UpIsceI | CGATTACCCTGTTATCCCTACCGGAAGGAGTCTTCCTGTTTATGG | ||
| D24607DnNotI | GAATGCGGCCGCCAAGTGGTTCCTGGACCGAATGGC | Downstream fragment of YALI0D24607 | |
| D24607Dn2IsceIIceuI | GGTAGGGATAACAGGGTAATCGTAACTATAACGGTCCTAAGGTAGCGAGGCAGGTATTAGATTTATATGAGTAGACG | ||
| Ver1D24607 | CGACATTTGAAGGAGATGACGG | Verification of disruption | |
| Ver2D24607 | GGACATTGTGCCCTGGGCCACC | ||
| StartD24607 | ATCGGATCCCACAATGACCCAGTCGTACGAAGTCGGAAAC | Overexpression of YALI0D24607 | |
| EndD24607 | CATCCTAGGCTAATGAACACTTCCAACAGTGGTCTG | ||
| HG-RT-D24607F2 | CCGGAGGAATCTTTATGG | Expression of YALI0D24607 by RT-PCR | |
| HG-RT-D24607R2 | GTGAGATGGATGGGGATG | ||
| YALI0E32901g (YLJEN6) | E32901UpNotI | GAATGCGGCCGCAGAGAGTTCTTTATCCACCCCACGG | Upstream fragment of YALI0E32901 |
| E32901UpIsceI | CGATTACCCTGTTATCCCTACCGGACTAGTGAGTGCTTGCCACGAG | ||
| E32901DnNotI | GAATGCGGCCGCCAGTACTCATTACCAGGCAATACGG | Downstream fragment of YALI0E32901 | |
| E32901Dn2IsceIIceuI | GGTAGGGATAACAGGGTAATCGTAACTATAACGGTCCTAAGGTAGCGACCAATATGACTCCCCCTACGAGTCC | ||
| Ver1 E32901 | CTCAACAAAGAGATGATAAGCC | Verification of disruption | |
| Ver2 E32901 | CTGTATTGAATATTTGACTGCTCC | ||
| StartE32901 | ATCGGATCCCACAATGGAAGCTCCTAATCTCTCGCCAGC | Overexpression of YALI0E32901 | |
| EndE32901 | CATCCTAGGTTACTTGGACTCGTAGGGGGAGTC | ||
| E32901F | CGGCTCTCAAGACCTCTAC | Expression of YALI0E32901 by RT-PCR | |
| E32901R | GAGCACCTCCAATAACACAA | ||
| pTEF-Start | GGGTATAAAAGACCACCGTCC | Verification of insertion of overexpression cassettes | |
| 61 Stop | GTAGATAGTTGAGGTAGAAGTTG | ||
| Actin | ACT-A1 | TCCAGGCCGTCCTCTCCC | Expression of Actin by RT-PCR |
| ACT-A2 | GGCCAGCCATATCGAGTCGCA |