Figure 8.

CopM is localized in both periplasmic and extracellular space. (A) Western blot analysis of CopM in cellular and extracellular fractions. WT cells were grown in BG11C-Cu medium to mid-log growth phase and exposed for 24 h to copper 1 μmol/L. Five micrograms of cytosol + periplasm or extracellular protein from soluble extracts was separated by 15% SDS-PAGE and subjected to western blot to detect CopM and TrxA as loading control. (B) Quantification of CopM levels in response to copper in cellular and extracellular fractions. Western blot signal of three independent experiments were quantified using ImageJ program. Error bars represent SE.