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Nested single cell PCR of differentiated GFP-positive cells. Representative gene expression of single new GFP-positive cells showed that these cells expressed insulin 1 (as would be expected with GFP-positive). Most also expressed insulin 2 (20/24) and the duct marker Ck19 (21/24). Only some cells had detectable levels of Pdx1 (10/24). Manually dissected duct tissue for CK19 and sorted GFP-positive beta cells from MIP-GFP mice served as positive control.
