Figure 3.

Knockdown of EphB4 blocked erythropoietin (EPO)–induced osteoblastic activity. ST2 cells were transfected with mouse EphB4 shRNA and the corresponding controls with Lipofectamine 2000. After 24 h, cells were cultured in osteogenic medium with EPO and/or ephrinB2-Fc. RNA was extracted on day 4, and expressions of EphB4, Runx2, Sp7, and Col1 in ST2 cells were quantified by quantitative reverse transcription polyermerase chain reaction. Data shown are mean ± SD. These assays were repeated 3 times. ^*P < 0.05; ^**P < 0.01.