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. Author manuscript; available in PMC: 2015 Aug 19.
Published in final edited form as: Nature. 2014 Dec 10;518(7539):413–416. doi: 10.1038/nature13981

Extended Data Figure 5. αKG increases Tet activity in mESCs.

Extended Data Figure 5

a, Simplified schematic of the reaction mechanism of TET1/2 enzymes. b, Relative percent 5-methylcytosine (% 5-mC) in ESC-1 cells cultured in S/L medium with or without DM-αKG for 24 hours. Each data point represents a sample from triplicate wells of a representative experiment. c, Gene expression in ESC-1 cells cultured with DM-αKG or DM-succinate for three days. d, Gene expression in ESC-1 cells cultured in S/L medium with or without DM-αKG for four passages. e, Gene expression in wild-type or Tet1/Tet2 double knock out (KO) mESCs cultured with DM-αKG or DM-succinate for 72 hours. qRT-PCR data (c–e) was normalized to Actin or Gapdh and samples were normalized to the control group. Oct3/4 is not expected to change and is included as a control. Data are presented as the mean ± s.e.m. of triplicate wells.