| Gene | Forward primer 5′ - 3′ | Reverse primer 5′ - 3′ |
|---|---|---|
| Pou5f1 | acatcgccaatcagcttgg | agaaccatactcgaaccacatcc |
| Nanog | aagatgcggactgtgttctc | cgcttgcacttcatcctttg |
| Esrrb | tttctggaacccatggagag | agccagcacctccttctaca |
| Klf2 | taaaggcgcatctgcgtaca | cgcacaagtggcactgaaag |
| Nr0b1 | tccaggccatcaagagtttc | atctgctgggttctccactg |
| Fgf5 | aaactccatgcaagtgccaaat | tctcggcctgtcttttcagttc |
| Zfp42 | cgagtggcagtttcttcttgg | cttcttgaacaatgcctatgactcacttcc |
| Actin | tggcgcttttgactcaggat | gggatgtttgctccaaccaa |
| Asz1 | gagtgggcttctcccagaaa | ggtcattttcccgctcattc |
| Wdfc15a | tgtgtggaaccctggacaac | gccaatgccgtcgttatttt |
| Dazl | caactgttaactaccactgcag | caagagaccactgtctgtatgc |
| Gapdh | ttcaccaccatggagaaggc | cccttttggctccaccct |
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