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. Author manuscript; available in PMC: 2016 Feb 18.
Published in final edited form as: Neuron. 2015 Jan 29;85(4):833–846. doi: 10.1016/j.neuron.2014.12.065

Figure 4.

Figure 4

Context-dependent regulation of Tmem100 in the TRPA1-V1 complex. (A)(B) MO (10 μM)- (A) and CAP (100 nM)-induced (B) single-channel open probability (Po) of the main conductance at Vh=-60 mV in CHO cells expressing TRPA1 vs. TRPA1+Tmem100 (T100) (1:4 molar ratio) and TRPA1+TRPV1 (1:1) vs. TRPA1+TRPV1+Tmem100 (1:1:4). The configuration is cell-attached patch, and the number of cells that responded among those analyzed is indicated within bars. The statistic is one-way ANOVA with Bonferroni’s post-hoc test for comparison between all columns (**p<0.01; ***p<0.001); error bars represent SEM. (C)(D) Representative single-channel recording during 5 sec at Vh=−60mV for MO-gated current (C) and 4 sec for CAP-gated current (D) in TRPA1+TRPV1− and TRPA1+TRPV1+Tmem100-expressing CHO cells; c is the closed state, o1, o2, and o3 show open states for 3 independent channels in the patch. (E) Summary of data on single-channel conductance (pS) and effects on Po changes of Tmem100 (T100) for the given agonists and genes expressed. The values of single-channel conductance were derived from Figure S4. Data are presented as mean ± SEM.