FIG. 2.

Experimental setup. (a) Overall system architecture of the platform. It includes four key modules: optogenetic illumination for neural manipulation, computer vision for illumination pattern alignment, mechanical actuation for C. elegans tracking, and post processing for offline force measurement. The blocks of the same module are labeled by identical color. (b) Schematic experimental setup of the platform. Note the microscope condenser provides bright-field light, the projector provides optogenetic light, the motorized stage actuates tracking of C. elegans, and the computer controls the hardware elements (e.g., camera, stage, and projector) and relevant image processing software.