Table 1.
Amplified sequence | Forward primer | Reverse primer | Product size (bp) |
---|---|---|---|
Entpd2 WT | gttagggggtgtctgtctgg | aaatgctgcctggaaatgg | 309 |
neomycin cassette | ccgtaaagcacgaggaagc | ctggacgaagagcatcagg | 263 |
Entpd2 KO | tcctgtcatctcaccttgctcctgc | gtttaagtgttgctctctgaccaactggc | 3904 |
P2ry1 WT | acagtactgtcgcctcaactgcagcagttt | ccgaagatccagtcagtcttgttgaagtag | 290 |
P2ry1 KO | gccttctatcgccttcttgacgagttcttc | ccgaagatccagtcagtcttgttgaagtag | 660 |