Fig. 4.

Expression of P2Y receptor mRNA obtained from neonatal rat cardiac myofibroblasts. Total RNA was prepared and reverse transcription-polymerase chain reaction was carried out; 1.5 % agarose gel electrophoresis represent mRNA coding for β-actin (lanes 1–2), P2Y₁ (lanes 3–4), P2Y₂ (lanes 5–6), P2Y₄ (lanes 7–8), P2Y₆ (lanes 9–10), P2Y₁₂ (lanes 11–12), P2Y₁₃ (lanes 13–14) and P2Y₁₄ (lanes 15–16). Lanes 2, 4, 6, 8, 10, 12, 14, 16 correspond to the primer control without cDNA and lane L to the ladder. Gel images presented are from one experiment and representative of seven independent experiments (reproduced from [291], with permission from The British Pharmacological Society)