Fig. 3.

Ca²⁺ mobilization of embryonic cardiomyocytes using FLIPR. The Ca²⁺ mobilization response is represented by an increase in fluorescence. a, b show responses induced by BzATP (100 μM) in E14 and E18 cardiomyocytes, respectively. c, d Responses induced by α,β-meATP (100 μM) in E14 and E18 cardiomyocytes, respectively (the control data for Fig. 3 are shown in Fig. 1). Representative tracings from three independent experiments are shown performed with three different embryos each day of experiment