Fig. 7.

P2X receptor expression in cultured E18 cardiomyocytes. Cardiomyocytes were identified by a monoclonal antibody against α-sarcomeric actin (α-SMA, green) (a, d, g, j, m). P2X receptor-expressing cells are shown in red (P2X2 (b), P2X4 (e), P2X1 (h), P2X5 (k) and P2X7 (n)). P2X receptor subtypes co-expressing α-SMA on cardiomyocytes are shown in yellow (c, f, i, l, o). P2X3 and P2X6 receptors were not expressed in E18 cardiomyocytes (data not shown). Scale bar = 50 μm. Images representative of n = 2 independent experiments, with at least three different embryos