Fig. 4.

The frequencies of mosaic clones in various genetic backgrounds. a The incidence of wts ^x1 GFP clones in adult AdoR ^RNAi and control flies. The UAS transgenes were expressed by MARCM system. The left bar (AdoR+) y w; act-Gal4 UAS-GFP/+ +; wts ^x1/tub-Gal80; the right bar (AdoR ^RNAi) y w; act-Gal4 UAS-GFP/+ +; UAS-AdoR ^RNAi-VDRC wts ^x1/tub-Gal80. Error bars show 95 % CI. The numbers above each bar indicate the number of flies tested. b AdoR and Ent2 overexpression and Ent2 silencing affect the frequency of wts ^x1 clones. The wts ^x1 clone frequencies, in flies with UAS-AdoR ⁺ (black bar, left), UAS-Ent2 ⁺ (left gray bar) and UAS-Ent2 ^RNAi-VDRC (right gray bar) constructs expressed using MARCM system (see the text). For comparison, wts ^x1 clone frequency observed in homozygous AdoR ⁺ flies is shown on the left (white bar). Error bars show 95 % CI. The numbers above each bar indicate the number of flies tested. c The incidence of dco ³ and control mosaic clones in AdoR ⁺ and AdoR ¹ flies. Black bars (left) the frequency of dco ³ clones in heterozygous dco ⁺/dco ³ flies (Act-Gal4 UAS-GFP/+ +; + AdoR ¹ dco ³/Tub-Gal80 + + and Act-Gal4 UAS-GFP/+ +; + dco ³/Tub-Gal80 +); gray bars (right) control GFP clones labeled by MARCM system in AdoR ¹ and AdoR ⁺ flies (Act-Gal4 UAS-GFP/+ +; + AdoR ¹/Tub-Gal80+ and Act-Gal4 UAS-GFP/+ +; +/Tub-Gal80). Error bars show 95 % CI. The numbers above each bar indicate the number of flies tested. d The ectopic expression of adenosine deaminase ADGF-A rescues wts ^x1 AdoR ¹ clones in UAS-ADGF-A/en-Gal4; wts ^x1 AdoR ¹/++ flies. The en-Gal4 was used to drive UAS-ADGF-A ⁺ expression in the posterior wing (gray shading in the schematic wing drawing above the graph indicates the en expression pattern). The hyperplastic outgrowth clone rates in the anterior and posterior compartments of 1518 wings examined were 0.2 ± 0.1 (left empty bar) and 2.7 ± 0.1 (right gray bar), respectively