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. 2015 Feb 20;10(2):e0117115. doi: 10.1371/journal.pone.0117115

Fig 6. Transcriptional abundance of cyp51 genes in HS-F6 wild-type and Δsre strains after prochloraz treatment.

Fig 6

The wild-type and Δsre strain were treated with or not with prochloraz (7μg/ml) and shaking at 25°C for 6 hours. Total RNA was isolated for qRT-PCR as described in Materials and methods. A: Bars represent the relative expression levels of CYP51 genes in HS-F6. B: Bars represent the relative expression levels of CYP51 genes in the ΔsreA strain; mRNA abundance was normalized by the housekeeping gene β-actin. The relative expression levels were calculated using the 2-ΔΔCt method. Three biological replicates were performed. (*P<0.05)