Table 1. Primers used in this study.
| Name | Sequence(5’-3’) | Purpose |
|---|---|---|
| sreA-a | ATTTGAACTACAAAGACTTCTC | PCR primers used to amplify the DNA fragment of sreA. |
| sreA-b | TACCACTCTCGGAAGAACCTATG | PCR primers used to amplify the DNA fragment of sreA. |
| sreA-c | GCCGGTCTGATGGTTCTTGAAGG | PCR primers used to amplify the DNA fragment of sreA. |
| sreA-d | TCCAATGAGAGAAAGCTGGACTGG | PCR primers used to amplify the DNA fragment of sreA. |
| sreA-e | ACACGAGGCCTAAGTTTTGTTGCTG | PCR primers used to amplify the 5’ unknown DNA sequence of sreA. |
| sreA-f | ATCAAGCCGACCGGAAGATTAGGC | PCR primers used to amplify the 5’ unknown DNA sequence of sreA. |
| sreA-g | TTGATCTCCTTCCGAGAACATGGGC | PCR primers used to amplify the 5’ unknown DNA sequence of sreA. |
| sreA-h | ATACTGGGCCCGAAATGCCTACACC | PCR primers used to amplify the 3’ unknown DNA sequence of sreA. |
| sreA-i | TAAGAAATACAGGACCCGTCGACGC | PCR primers used to amplify the 3’ unknown DNA sequence of sreA. |
| sreA-1 | CCCTCGAGATGTCTGGCCCCAATATGGAG | PCR primers used to amplify 5’ fragments of sreA. |
| sreA-2 | GGACTAGTCAGCAAAGACTCCATGGTTTGC | PCR primers used to amplify 5’ fragments of sreA. |
| sreA-3 | CGAGCTCTTTCGAAGCAAGCGAGAAGG | PCR primers used to amplify 3’ fragments of sreA. |
| sreA-4 | GGGGTACCTCAGGCAGGGACATTTTGCA | PCR primers used to amplify 3’ fragments of sreA. |
| sreA-F | ATGGATGTCTGGCCCCAATATGGAG | PCR primers used to amplify the ORF of sreA gene. |
| sreA-R | TCAGGCAGGGACATTTTGCAC | PCR primers used to amplify the ORF of sreA gene. |
| sreA-F1 | GGACTAGTGGCAATAGTGGAGACTA GCAC | PCR primers used to amplify sreA, including its promoter and terminator. |
| sreA-R1 | GGACTAGTCTGATAACATTCCATTTCCC | PCR primers used to amplify sreA, including its promoter and terminator. |
| cyp51A-F | CACTGGATTCCTTTCATTGGG | PCR primers used to amplify cyp51A gene by quantitative real-time PCR. |
| cyp51A-R | TCCGAAGACGGGGGTTGTAA | PCR primers used to amplify cyp51A gene by quantitative real-time PCR. |
| cyp51B-F | GAGTTCATCCTCAATGGCAAGC | PCR primers used to amplify cyp51B gene by quantitative real-time PCR. |
| cyp51B-R | CTTAGAGTTGGGGCAATCGTAGAC | PCR primers used to amplify cyp51B gene by quantitative real-time PCR. |
| cyp51C-F | TGTTCAAGCAGCCATTCAAGC | PCR primers used to amplify cyp51C gene by quantitative real-time PCR. |
| cyp51C-R | CAAGTTGGGTCCGACGAAATA | PCR primers used to amplify cyp51C gene by quantitative real-time PCR. |
| β-actin-F | TGTCACCAACTGGGACGATA | PCR primers used to amplify β-actin gene by quantitative real-time PCR. |
| β-actin-R | GAGCTTCGGTCAAGAGGATG | PCR primers used to amplify β-actin gene by quantitative real-time PCR. |
Note: The underlined sequences represent different restriction enzyme sites.