FIG 3.

Germination extents and PC image intensities of individual wild-type B. subtilis spores germinating with l-valine at various temperatures. B. subtilis PS533 (wild-type) spores were germinated with 10 mM l-valine at various temperatures, and spore germination was monitored by PC image intensity changes as described in Materials and Methods. The PC image intensities (a.u.) were normalized to 1 based on the respective values at the first time of measurement, and PC image intensities at the end of the experiment were set at 0. The arrows indicate the times of T₁, T_lag, T_release, and T_lysis for a single spore. In panel a, the germination curves at the different temperatures are from data with >262 spores each, and a germinated spore was defined as one that had reached T_release. In panel d, “s” means spores germinating slowly at 45°C (T_release of >16.0 min in panel a).