TABLE 3.
Mean values and standard deviations of germination parameters of multiple individual ↑SpoVA and cwlJ B. subtilis spores germinating with l-valine at different temperaturesa
| Spore strain | Germination temp (°C) | Mean ± SD |
No. of germinated spores (% germination) | |||||
|---|---|---|---|---|---|---|---|---|
| T1 (min) | Tlag (min) | ΔTleakage (min) | Trelease (min) | ΔTrelease (min) | Ilag | |||
| ↑SpoVA | 25 | 3.9 ± 3.8 | 7.9 ± 7.7 | 4.0 ± 5.5 | 11.8 ± 8.4 | 3.9 ± 1.8 | 0.89 ± 0.11 | 354 (98.3) |
| 37 | 3.8 ± 3.0 | 8.0 ± 7.9 | 4.2 ± 6.5 | 11.2 ± 8.6 | 3.1 ± 1.3 | 0.88 ± 0.09 | 335 (98.9) | |
| 45 | 3.8 ± 3.0 | 7.4 ± 7.7 | 3.6 ± 6.3 | 10.6 ± 8.3 | 3.1 ± 1.3 | 0.87 ± 0.10 | 321 (99.3) | |
| FB111 (cwlJ) | 25 | 20.0 ± 11.1 | 72.2 ± 25.6 | 52.2 ± 19.9 | 81.1 ± 27.1 | 8.9 ± 3.5 | 0.48 ± 0.07 | 360 (93.5)b |
| 37 | 8.2 ± 4.7 | 34.1 ± 12.3 | 25.9 ± 10.4 | 38.9 ± 12.8 | 4.8 ± 1.3 | 0.57 ± 0.07 | 432 (94.5)c | |
| 45 | 8.1 ± 5.7 | 24.7 ± 9.1 | 16.6 ± 6.9 | 28.4 ± 9.4 | 3.7 ± 0.84 | 0.61 ± 0.07 | 316 (97.8)c | |
a
As described in the legend to Fig. 5, PS3411 (↑SpoVA) spores were germinated with 2 mM l-valine at various temperatures, and spore germination parameters were determined as described in Materials and Methods. As described in the legend to Fig. S2 in the supplemental material, FB111 (cwlJ) spores were germinated with 10 mM l-valine at various temperatures, and spore germination parameters were determined as described in Materials and Methods.
b
Germination was for 2 h.
c
Germination was for 1 h.