TABLE 2.
Inhibition of radioactive precursor incorporation into E. coli macromolecular synthesis pathways
| Compoundb | Target | Incorporation IC50 (µg/ml)a for: |
||||
|---|---|---|---|---|---|---|
| Protein [14C]leucine | Cell wall [3H]DAP | Fatty acid [14C]acetic acid | RNA [3H]uridine | DNA [3H]thymidine | ||
| Erythromycin | Protein synthesis | 1.3 | >256 | >256 | >256 | >256 |
| Ampicillin | Cell wall synthesis | >256 | 49 | >256 | >256 | >256 |
| Triclosan | Fatty acid synthesis | 1.3 | 0.8 | 0.007 | 0.08 | 1.3 |
| Rifampin | Transcription | 16 | >256 | >256 | 7 | >256 |
| Ciprofloxacin | DNA replication | 0.6 | >256 | >256 | 0.2 | 0.02 |
| CCCP | Membrane potential | 0.3 | 0.04 | 0.02 | 0.03 | 0.1 |
| Compound 1 | Cell wall synthesis | >67 | 0.2 | >67 | >67 | >67 |
a
Incorporation of radiolabeled precursors was measured in E. coli ΔtolC ΔlysA cells.
b
CCCP, carbonyl cyanide m-chlorophenylhydrazone.