FIG 4.
Inactivation of PpsA's ACPL domain leads to a PGL− PDIM− phenotype in M. marinum. Radio-TLC analysis of 14C-labeled PGLs (A) and 14C-PDIMs (B) from M. marinum wt + pCP0 (lane 1), M. marinum ppsAS-A + pCP0 (lane 2), and M. marinum ppsAS-A + pCP0-ppsA (lane 3). The wild-type (wt) and mutant M. marinum strains carried the vector pCP0 so they could be cultured in the same kanamycin-containing medium used for the complemented M. marinum ppsAS-A + pCP0-ppsA strain. TLC solvent systems used are indicated. CHCl3, chloroform; MeOH, methanol; PE, petroleum ether; Et2O, diethyl ether.