FIG 6.

Extension of a synthetic PHPA substrate by the M. marinum FadD29-PpsA in vitro system. Extracted ion chromatogram and isotopic distributions for the synthetic PHPA substrate (s-PHPA) and the extended s-PHPA product (e-s-PHPA) in the complete reaction mixture (A) and extracted ion chromatograms from two representative negative-control reaction mixtures, i.e., one containing no malonyl-CoA (B) and the other no s-PHPA (C). The retention time (RT) is indicated in the chromatograms. The dashed-line boxes in the observed isotopic distributions indicate the calculated relative species abundance, which is in agreement with the experimental data.