Table 2.
Gene | Location | Polymorphism | Primers/Probes |
---|---|---|---|
IL10 | Promoter | SNP −592 C>A (rs1800872) | VIC/FAM: CTTTCCAGAGACTGGCTTCCTACAG[G/T]ACAGGCGGGGTCACAGGATGTGTTC |
IL10 | Promoter | SNP −819 C>T (rs1800871) | Fw: 5′ GGCACTGGTGTACCCTTGTA 3′ |
Rv: 5′ CATGACCCCTACCGTCTCTATTTT 3′ | |||
FAM: 5′ AGGCACAGAGATATTACAT 3′ | |||
VIC: 5′ AGGCACAGAGATGTTACAT 3′ | |||
IL10 | Promoter | SNP −1,082 A>G (rs1800896) | VIC/FAM: TCCTCTTACCTATCCCTACTTCCCC[T/C]TCCCAAAGAAGCCTTAGTAGTGTTG |
SNP, single nucleotide polymorphism; Fw, forward primer; Rv, reverse primer.
Taqman probes are listed with the polymorphic bases in bold.