Fixed protein concentration and changes in surface/volume ratio underlie domain scaling. (A, C, and E) DIC images representing the median embryo of each recording condition and (B, D, and F) corresponding kymographs, overlaid with landmarks similar to Fig. 1. The white dashed line in E delimits the boundary of the raw image. (G) Linear regression (dark gray line) performed on the length of the embryo versus the fraction of the membrane spanned by GFP::PAR-2, overlaid with landmarks similar to Fig. 3. The darker circles indicate the locations of the median recordings depicted in (A)–(F). (H) Same as (G), but quantifying the extent of the posterior domain as predicted by M3 (i.e., using the values calibrated with the median recordings). To see this figure in color, go online.