Figure 5.

Diffusion is the process most affected by temperature. (A–C) Average kymographs of polarity establishment at the indicated temperatures, overlaid with landmarks similar to Fig. 1. Arrows: portion of the maintenance stage (white line) averaged for the profiles displayed in (E). (D) Quantification of duration of the maintenance phase (see the Supporting Material) at the three temperatures, overlaid with tests of significance (Student’s two-tailed t-test). (E) Profiles of GFP::PAR-2 signal during maintenance (arrows in A–C) at different temperatures. Thin lines: individual profiles; thick lines: average of each condition. Each set of curves (i.e., each recording temperature) is statistically different from each other (p < 0.001, adaptive Neyman test (40)). Arrowheads: variations predicted by the model (see G). (F) Quantification of the time required for polarity maintenance as in (D) when the values of the diffusion parameters D_A and D_P are scaled by the factor λ. Note that the x axes in (D) and (F) cannot be directly compared; however, a similar trend is observed. (G) Profiles simulated by the model as in (F) and aligned as in (E). To see this figure in color, go online.