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. 2015 Feb 17;108(4):893–902. doi: 10.1016/j.bpj.2014.12.028

Table 2.

Interlabel distances and distribution widths of doubly labeled mini TAR DNA

Sample 〈rDEER〉 (Å)a W1/2 (Å)a
SLAB by itself 27.5 ± 0.5 7.0 ± 0.5
SLCD by itself 26.8 ± 0.4 5.2 ± 0.4
SLEF by itself 26.3 ± 0.4 4.3 ± 0.4
1:1:4 SLCD/mini TAR RNA/NCp7 35.1 ± 0.6 10.8 ± 1.0
1:1:4 SLEF/mini TAR RNA/NCp7b 34.8 ± 0.6 14.0 ± 1.0
1:1:4 SLAB/mini TAR RNA/NCp7 76.5 ± 2.0 19.5 ± 2.0
a

〈rDEER〉 and W1/2 are the respective experimental interlabel distance and the full peak width at half height of the interlabel distance distribution, as determined experimentally by DEER.

b

In the presence of a fourfold excess of NCp7 with respect to mini TAR DNA and mini TAR RNA, there is evidence for a broad shoulder in the interlabel distribution that extends above 50 Å separation; it is most notable in the SLEF sample.