Figure 3.

Dependence of dynein-driven corkscrewing motion on ATP concentration. (A) Microtubule rotational pitches driven by outer-arm dynein and its subparticles are plotted at various ATP concentrations. Data points and error bars are mean pitch and standard deviation respectively of the corkscrewing microtubule, driven by αβγ (circle), βγ (square), and α (triangle), at 1, 3, 10, 30, and 100 μM ATP concentration. The xyz position of the QDs bound to microtubules was tracked every 0.05–2 s. Sliding velocity (B) and rotational velocity (C) are plotted against ATP concentration.