Semi-log plot showing the equilibrium binding of Kapβ1 (open circles), NTF2 (solid circles), and W7A-NTF2 (triangles) to Nsp1p-12FF brushes. The data were normalized by the maximum binding capacity (fraction of saturation) and are shown as a function of injected bulk NTR concentration. Solid lines represent the average two-component Langmuir isotherm for Kapβ1, NTF2, and W7A-NTF2, respectively. To see this figure in color, go online.