Table 4.

Relative gene expression of five selected genes tested in this study with presporogonic stages, intra-luminal sporogonic stages, and relative parasite load

Genes		Different time points
		6 wpe		8 wpe		10 wpe		12 wpe
		BT	RT	BT	RT	BT	RT	BT	RT
	Presporogonic parasite stages	Low	Moderate	Low	High	Moderate	High	Low	Moderate
	Intra-luminal sporogonic stages	Very few	ND	High	ND	High	ND	Moderate	ND
	Relative parasite load	Low	High	High	High	High	High	High	Moderate
TSC22D3	R.E.	0.768	1.475	1.588	0.598	1.234	0.491	1.897	0.554
	p value	0.006		0.0001		0.0001		0.001
IgM H	R.E.	3.984	5.742	12.815	7.201	2.404	9.757	1.840	2.994
	p value	0.004		0.003		0.002		0.050
Rab-11b	R.E.	0.349	1.339	0.542	0.651	0.346	1.80	0.478	0.344
	p value	0.0001		0.366		0.0001		0.089
PTMA-α	R.E.	1.116	1.096	1.354	0.615	0.745	0.832	1.803	0.623
	p value	0.869		0.001		0.191		0.0001
Beta thymosin	R.E.	0.850	1.342	1.083	1.386	1.176	1.702	0.994	1.150
	p value	0.004		0.013		0.012		0.267

Normalized mean values of relative gene expression (R.E.) in the kidney of infected brown trout (BT) and infected rainbow trout (RT) are presented with p values at different time points. The differences between groups at each single time point were analyzed using t tests for independent samples with Bonferroni α-correction

ND not detected