Table 2.
Thermal cycle protocol used for both dPCR and ddPCR protocols
| Name | Phase | Time [s] | Temperature [°C] | Repeats |
|---|---|---|---|---|
| UNG and hot start | UNG | 120 | 50 | 1 |
| Hot start | 600 | 95 | ||
| PCR cycles | Denaturation | 15 | 95 | 50 |
| Annealing | 60 | 60 |
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