Table 5.

Average volume of droplets generated by the Bio-Rad Droplet Generator using either Supermix for Probes or EvaGreen Supermix. The uncertainty on the average copy number concentration is an expanded uncertainty with a coverage factor k of 2

Cartridge	Mastermix	Droplet volume (nL)	Total number of droplets
DG8 (production year 2012)a	ddPCR™ Supermix for Probes	0.833 ± 0.035	622
DG8 (lot nos. C000021590, C000024241 and C000031616)a	QX200™ ddPCR™ EvaGreen® Supermix	0.830 ± 0.027	805
DG8 (production year 2013)b	ddPCR™ Supermix for Probes	0.860 ± 0.021	463

^aDroplets were analysed from three wells from each of five DG8 cartridges (15 wells in total)

^bSixteen 2 μL samples were collected sequentially from droplets generated from a single well of a DG8 cartridge and the average droplet volume measured