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. 1973 Jul;70(7):1973–1975. doi: 10.1073/pnas.70.7.1973

Effects of Selective Extraction of Histones on Template Activities of Chromatin by Use of Exogenous DNA and RNA Polymerases

Alfred E Mirsky 1, Bert Silverman 1
PMCID: PMC433645  PMID: 4579008

Abstract

Exogenous DNA and RNA polymerases were used to measure the template activity of DNA in chromatin in isolated thymus nuclei from which lysinerich or arginine-rich histones were selectively extracted. Measurements were on nuclei containing 20-1200 μg of DNA per ml, the distinctions becoming clear at the higher concentrations. Experiments with RNA polymerase showed only moderate increases in template activity upon extraction of histone, although the removal of lysinerich histone had a greater effect than that of arginine-rich histone. DNA polymerase action on nuclei minus lysinerich histone achieved high results, exceeding even those on DNA itself.

Keywords: DNA polymerase, RNA polymerase, isolated thymus nuclei, histones

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1973

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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