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. 2015 Jan 23;14:9. doi: 10.1186/s12934-014-0189-x

Table 2.

Comparison of the redox self-sufficent amination of 1,10-decanediol by W3110/pTrc99a- ald-adh-ta Vf and W3110/pTrc99a- ald-adh-ta Cv with varying L-alanine concentrations

Strain Biotransformation conditions Reaction temperature [°C] Max. conversion [%] Alanine consumption [mM]
W3110/pTrc99a- ald-adh-ta Cv 100 mM alanine, 100 mM NH4Cl 37 10012h 30
50 mM alanine, 100 mM NH4Cl 8412h 30
20 mM alanine, 100 mM NH4Cl 868h 16
W3110/pTrc99a- ald-adh-ta Vf 100 mM alanine, 100 mM NH4Cl 37 10024h 30
50 mM alanine, 100 mM NH4Cl 7312h 26
20 mM alanine, 100 mM NH4Cl 5012h 18