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. 2015 Feb 7;13:7. doi: 10.1186/s12953-015-0064-7

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© Seaver and Livingston; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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A Maximum Likelihood tree showing the relationships among O. wendtii and S. purpuratus spicule matrix proteins found in the skeletal proteomes or identified through BLAST searches of the S. purpuratus genome using O. wendtii proteins as query. Alignments are shown in Additional file 6 with Gblocks identified. O. wendtii proteins are in bold. Boxed proteins are those not found in the proteome; all others are found in skeletal proteomes. Proteins with *are those that had expanded repeats removed prior to alignment. 1 indicates two splice variants of the same gene. Bootstrap values of 50 or greater are showhn in red. Nodes with bootstrap values below 25 were collapsed. The accession numbers for the sequences used are as follows: [Genbank: XP_796721.1, NP_999775.1, NP_999630.1, NP_999804.1, XP_003726194.1, NP_999803.1, NP_999776.1, XP_781636.1, KJ999723, KJ999724, KJ999725, KJ999726, KJ999727].