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. 1973 Jul;70(7):1998–2002. doi: 10.1073/pnas.70.7.1998

Restoration of Hypoxanthine Phosphoribosyl Transferase Activity in Mouse 1R Cells After Fusion with Chick-Embryo Fibroblasts

Bohdan Bakay *,†, Carlo M Croce ‡, Hilary Koprowski ‡, William L Nyhan *
PMCID: PMC433651  PMID: 4516198

Abstract

Fusion of the 1R mouse cell, which lacks activity of hypoxanthine phosphoribosyl transferase (EC 2.4.2.8), with chick-embryo fibroblasts yielded progeny cells that survived in hypoxanthine-aminopterin-thymidine selective medium. This property and the failure of the progeny to survive in 8-azaguanine indicated that hypoxanthine phosphoribosyl transferase activity was present. Electrophoretic analysis revealed that the enzyme was of mouse, not chick, origin. These observations are consistent with the operation of a regulator gene responsible for the absence of hypoxanthine phosphoribosyl-transferase activity in the 1R cell and its presence in the progeny.

Keywords: mouse-chick clones, enzyme activation, regulator gene

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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