Fig. 2.
Determinant for trans-interaction in the extracellular loop2 (ECL2) is conserved between Cld5 and Cld3. Three days after transfection of HEK cells with Cld3wt (a, b) or Cld3Y147A (c, d) cells were fixed, stained against Cld3 (red in a, c; white in b, d) and nuclei (blue in a, c) and enrichment at cell–cell contacts quantified using fluorescence intensity profiles (b, d; red box). The factor of contact enrichment (E F) was four times and the percentage of contacts showing an enrichment (PE) 6.5 times higher for Cld3wt than for Cld3Y147A (e, f). This indicates that the substitution Y147A inhibits trans-interaction of Cld3. Mean ± SEM; n = 123 for Cld3wt, n = 213 for Cld3Y147A; ***p < 0.001, compared to Cld3wt