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. 2014 Jul 7;16(11):1693–1705. doi: 10.1111/cmi.12319

Figure 1.

Figure 1

Wild type EHEC does not reduce SEAP secretion; overexpressed EspG reduces SEAP secretion and disrupts the Golgi.

A. SEAP secretion was measured from HeLa cells expressing SEAP and infected for 5 or 7.5 h with the indicated EHEC strains. The percentage secreted SEAP was normalized to uninfected cells from the 5 h infection time-point. Results are mean ± SEM from three independent experiments. ****P < 0.0001.

B. The expression (‘pellet’) and secretion (‘S/N’) of EspG was compared between the indicated EHEC strains grown under the conditions used for cell infection experiments. EspG and DnaK (loading control) were detected with specific antibodies to each.

C. Golgi integrity was assessed by staining HeLa cells infected with the indicated EHEC strains for 5 h with GM130 (cis-Golgi marker) and TGN46 (trans-Golgi network marker) and D. cis-Golgi and TGN dispersion was quantified as a percentage of the total area of the cell using ImageJ (35–40 cells per condition). *P < 0.05, ****P < 0.0001.