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. 2014 Jul 7;16(11):1693–1705. doi: 10.1111/cmi.12319

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© 2014 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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EspG localizes to recycling endosomes and the trans-Golgi network, altering their morphology.

A. Localization of EspG:HA was characterized by immunofluorescence analysis of recycling endosome markers (GFP:Rab11a, GFP:VAMP3 and endogenous TfR staining) or Golgi markers (endogenous TGN46 and GM130 staining). All cells were infected with ΔespG + pEspG:HA for 5 h and co-stained with DAPI and HA, representative images are shown.

B. TEM analysis of uninfected or infected cells incubated with Tf-HRP before fixation and DAB staining to reveal electron-dense Tf-positive vesicles. Representative images of the distribution of cellular organelles, including tubular recycling endosomes (RE), Golgi stacks (GS) or Golgi compartments (GC) are indicated.