Table 2.
Expression of Proteins by Dural Epithelial and Fibroblast Cells as Determined by Indirect Immunofluorescence
| Antibody specificity | Epithelial cells | Fibroblasts cells | Primary smooth muscle cells |
|---|---|---|---|
| Fibronectin | +ve | +ve | ------- |
| Tenascin | +ve | +ve | ------- |
| Collagen I | +ve | +ve | -------- |
| Collagen III | +ve | +ve | -------- |
| Actin | +ve | +ve | +ve |
| Glucose transporter (Glut-1) | +ve | +ve | +ve |
| Von Willebrand factor | +ve | −ve | −ve |
| E-Cadherin | +ve | −ve | --------- |
| CD-31 | +ve | −ve | −ve |
| Desmoplakin | +ve | −ve | −ve |
| Porcine endothelial cells | −ve | −ve | −ve |
| Human fibroblast/epithelial cells | −ve | −ve | ------- |
| Smooth muscle myosin, Heavy chain | −ve | −ve | +ve |
| Smoothelin | −ve | −ve | +ve |
| Desmin | −ve | −ve | +ve |
Primary smooth muscle cells were used as a positive control for smooth muscle cell contamination (+ve: positive staining, −ve: negative/no staining, ------: not tested).