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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jan 21;1849(3):317–327. doi: 10.1016/j.bbagrm.2015.01.003

Figure 4.

Figure 4

Effects of EGF on cellular sensitivity to mitoxantrone in IGROV1 cells. IGROV1 cells were plated at 2 × 104 cells/mL in 96-well plates in complete medium. Following starvation overnight in SFM, the cells were divided to four different groups: untreated (control), Fumitremorgin C (FTC, 10 μM, a specific inhibitor of BCRP), EGF (10 ng/mL), or EGF (10 ng/mL) plus FTC (10 μM). After 24 hours of EGF treatment, cells in each treatment group were continuously exposed to the indicated concentrations of mitoxantrone for 48 hours, after which survival was assessed by staining with SRB as described in Materials and Methods. Results are expressed as percent survival compared to cultures without mitoxantrone, presented as the mean of six individual values ± SD. *: Significantly different compared to no FTC by student's t-test, P=0.037; **: Significantly different compared to no EGF by Student's t-test, P<0.01.