Figure 2. Transcription initiation from divergent core promoters occurs at edges of open chromatin.
a, Normalized 5′-GRO-Seq (blue) read 5′end counts and DNaseI-seq (orange) read 5′end counts in bins of 10, ±0.5 kb from the center point of divergent TSS pairs (n = 3865; see Experimental Procedures), ranked from top to bottom by increasing distance between pairs. b, Genomic DNA sequence of divergent TSS pairs centered and ranked as in “a”. Bases ‘A’ and ‘T’ are yellow, bases ‘C’ and ‘G’ are blue. See also Figures S2 and S3. c, TATA-sensitive in vitro transcription of reverse directed core promoters. +1 TSS is marked by the arrow.