A, B. In NCM460 and HT-29 cells, marked decline in mRNA expression of CHST11 followed ARSB silencing (p<0.001, p<0.01; one-way ANOVA with Tukey-Kramer post-test; n=6).
C. In the ARSB-null mice, the Chst11 expression in the mouse colonic epithelium was significantly reduced, compared to wild-type C57BL/6J mice (p=0.001, unpaired t-test, two-tailed; n=6).
D, E. Sulfotransferase activity in the NCM460 and HT-29 cells was significantly reduced following ARSB silencing (one-way ANOVA with Tukey-Kramer post-test; n=6 for NCM460; n=3 for HT-29).
F. Sulfotransferase activity was significantly reduced in the ARSB-null mice, compared to the wild-type C57BL/6J mice (unpaired t-test, two-tailed; n=6).
G. Expression of CHST12 and CHST15 was unaffected in the ARSB-null mice (n=6).
[ARSB=arylsulfatase B; CHST11=carbohydrate (chondroitin 4) sulfotransferase 11; CHST12=carbodhydrate (chondroitin 4) sulfotransferase 12; CHST15=carbohydrate (N-acetylgalactsosamine 4-sulfate 6-O) sulfotransferase; F=female; M=male; si-small interfering siRNA]