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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Dec 12;1849(3):342–352. doi: 10.1016/j.bbagrm.2014.12.009

Figure 1. Expression of CHST11 and sulfotransferase activity are reduced when ARSB is silenced in human colonic epithelial cells and in ARSB-null mice.

Figure 1

A, B. In NCM460 and HT-29 cells, marked decline in mRNA expression of CHST11 followed ARSB silencing (p<0.001, p<0.01; one-way ANOVA with Tukey-Kramer post-test; n=6).

C. In the ARSB-null mice, the Chst11 expression in the mouse colonic epithelium was significantly reduced, compared to wild-type C57BL/6J mice (p=0.001, unpaired t-test, two-tailed; n=6).

D, E. Sulfotransferase activity in the NCM460 and HT-29 cells was significantly reduced following ARSB silencing (one-way ANOVA with Tukey-Kramer post-test; n=6 for NCM460; n=3 for HT-29).

F. Sulfotransferase activity was significantly reduced in the ARSB-null mice, compared to the wild-type C57BL/6J mice (unpaired t-test, two-tailed; n=6).

G. Expression of CHST12 and CHST15 was unaffected in the ARSB-null mice (n=6).

[ARSB=arylsulfatase B; CHST11=carbohydrate (chondroitin 4) sulfotransferase 11; CHST12=carbodhydrate (chondroitin 4) sulfotransferase 12; CHST15=carbohydrate (N-acetylgalactsosamine 4-sulfate 6-O) sulfotransferase; F=female; M=male; si-small interfering siRNA]