Fig. 4.

DCs treated with SOCS1 siRNA have enhanced capacity to activate T cells. Purified T cells were counted and seeded into 96-well plates at 10⁵ cells/well at room temperature with an equal volume of 2.5 μm CFSE. DCs were exposed to medium alone or SOCS1 siRNA, treated with C. albicans for 24 h, to stimulate T cells. Five hundred, 2,000, or 10,000 irradiated DCs were added to triplicate wells. After 5 days, the cells were collected and stained with PE-conjugated anti-CD4 mAbs for analysis. a The numbers in the upper left of each graph represent the percent of CD4⁺ T cells that was proliferating. b The results are expressed as the percent of CD4⁺ T cells at different ratios of DC-to-T cells. Mean ± SD of data from seven experiments is shown. **p < 0.01